General description

Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Note: 1 mU = 1 milliunit.

Native β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.

Packaging

500 miu in Plastic ampoule

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Reconstitution

Following initial use, refrigerate (4°C).

Unit Definition

One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.

Warning

Toxicity: Standard Handling (A)